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Image Search Results


Journal: Cell Systems

Article Title: Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis

doi: 10.1016/j.cels.2018.08.011

Figure Lengend Snippet:

Article Snippet: The following BrainPhys supplements were used for 10 ml media: 200 μl NeuroCultTM SM1 Neuronal Supplement (05711, Stemcell Technologies); 100 μl N2 Supplement-A (17502048, Thermo Fisher Scientific); 20 μl of 10 μg/ml recombinant human BDNF to a final concentration of 20 ng/ml (450-02, Peprotech); 20 μl of 10 μg/ml recombinant human GDNF to a final concentration of 20 ng/ml (450-10, Peprotech); 98 μl of 50 mg/ml dibutryl-cAMP to a final concentration of 1 mM (D0627, Sigma-Aldrich); 50 μl of 40 mM ascorbic acid to a final concentration of 200 nM (A0278, Sigma-Aldrich); and 100 μl of 100× penicillin-streptomycin (15140122, Thermo Fisher Scientific).

Techniques: Recombinant, Luciferase, cDNA Synthesis, Plasmid Preparation, Software